Molecular Diagnostics
Guidelines for Bovine Tritrichomonas foetus testing:
Specimen Handling, and Transport
Per the Arizona Department of Agriculture’s June 2020 revision of ARS3-1205, the only official trichomoniasis test for the purposes of importing, exporting, selling, or transferring of bulls is an individual PCR test submitted by a USDA accredited veterinarian.
Submit preputial samples from bulls in the InPouchTM TF culture system, TF-Transit Tube (Biomed Diagnostics) or PBS tube (if you need PBS tubes please call or email and we will provide those to you at no cost, only S&H fees will apply). Pouches can be ordered directly from the manufacturer at biomeddiagnostics.com. Include inoculation date on the tube. Do not use pouches past the expiration date. If samples are received in an expired pouch or tube, they can still be processed but a note will be added stating that the results cannot be used for the purposes of importing, exporting, selling, or transferring of bulls.
Hold inoculated pouches at room temperature until shipment to the lab. Protect the inoculated pouches from heat, cold, and direct sunlight.
Ship samples overnight. Never refrigerate or freeze inoculated pouches. Protect from extreme heat and cold. Use extra insulation and you can use a room temp ice pack in cold weather and refrigerated, NOT frozen, ice pack in hot weather. Samples must be kept between 59°F and 99°F from sample collection to arrival at the AZVDL. Exposure to temperature extremes can affect T. foetus growth and PCR results.
PBS tubes must be received within 96 hours after inoculation. Inoculated pouches must arrive at the lab within 48 hours (2 days) of collection as false negative results are possible. Samples received after 48 hours (pouches/transit tubes) and 96 hours (PBS tubes) can still be processed however a note will be added stating that the results cannot be used for the purposes of importing, exporting, selling, or transferring of bulls. Failure to follow the above guidelines may result in rejection of the sample at the discretion of the laboratory.
| Test Description | Test # | Price | Specimen Preferred | Turnaround Time |
|---|---|---|---|---|
| Bovine Herpesvirus Type-1 (IBR/BHV-1) PCR | M101 | $35.00 | 1-2mL EDTA whole blood, semen, transtracheal wash or bronchoalveolar lavage (BAL) | 2-3 working days |
| Bovine Parainfluenza-3 Virus PCR | M173a | $35.00 | Nasopharyngeal swab, nasal swab, 1-2mL transtracheal wash, or bronchoalveolar lavage, 1gram fresh or frozen lung or trachea |
2-3 working days |
| Bovine Viral Diarrhea PCR | M106 | $35.00 | Nasopharyngeal swab, 1-2mL EDTA whole blood, semen, transtracheal wash or bronchoalveolar lavage (BAL) | 2-3 working days |
| Bovine Viral Diarrhea (Pool) | M106a | $50.00 | Nasopharyngeal swab, 1-2mL EDTA whole blood, semen, transtracheal wash or bronchoalveolar lavage (BAL); ear notch (up to 20 samples per pool) | 2-3 working days |
| Bovine Resp. Syncytial Virus PCR | M168 | $35.00 | Nasopharyngeal swab, nasal swab, 1-2mL transtracheal wash or bronchoalveolar lavage (BAL) | 2-3 working days |
| Bluetongue Virus rt-PCR | M105 | $40.00 | 1-2 mL semen, 1-2mL EDTA whole blood, or 1 gram fresh spleen or lymph node. | 2-3 working days |
| Campylobacter Fetus subsp Venerialis PCR | M236 | $35.00 | 1-2 mL preputial wash, 1-2 mL cervical swab, 1-2g feces, 1 g fresh tissue (fetus, placenta, lung) | 2-3 working days |
| Canine Influenza Virus PCR | M242 | $35.00 | Tracheal or nasal swab, 1g fresh tracheal or lung, 1-2mL tracheal wash | 2-3 working days |
| Canine Herpesvirus PCR | M109 | $35.00 | Fresh tissue, nasal swab, tracheal swab, pharyngeal swab, conjunctival swab. | 2-3 working days |
| Canine Parvovirus PCR | M110 | $35.00 | Fresh feces, 1 mL intestinal content | 2-3 working days |
| Elephant Endotheliotropic Herpesviruses PCR | M248 | $75.00 | 1-2mL EDTA whole blood, trunk wash | 1-2 working days |
| Equine Herpesvirus Type 1 PCR | M113a | $35.00 | 1-2mL EDTA whole blood, nasal swab, transtracheal wash or bronchoalveolar lavage (BAL) | 2-3 working days |
| Equine Herpesvirus Type 1 & 4 PCR | M113 | $45.00 | 1-2mL EDTA whole blood, nasal swab, transtracheal wash or bronchoalveolar lavage (BAL) | 2-3 working days |
| Epizootic Hemorrhagic Disease PCR | M111 | $40.00 | 1-2 mL semen, 1-2mL EDTA whole blood, or 1 gram fresh spleen or lymph node. | 2-3 working days |
| EHD and BTV Multiplex rt-PCR | M194 | $60.00 | 1-2 mL semen, 1-2mL EDTA whole blood, or 1 gram fresh spleen or lymph node. | 2-3 working days |
| Feline Parvovirus PCR | M118 | $35.00 | Fresh feces, 1 mL intestinal content, swab in transport media | 2-3 working days |
| Malignant Catarrhal Fever PCR | M121 | $35.00 | 3 mL pharyngeal wash, nasal secretion, nasopharyngeal swab | 2-3 working days |
| SARS Coronavirus-2 (COVID-19) PCR | M163 | $70.00 | Nasal swab, oropharyngeal swab, rectal swab, 1-2g feces | 2-3 working days |
| Streptococcus Equi PCR | M158 | $35.00 | 1-3 mL pharyngeal wash, nasal secretion, nasopharyngeal swab | 2-3 working days |
|
Tritrichomonas Foetus PCR Tritrichomonas Pooled Samples |
M143 |
$22.00 $40.00 |
In-Pouch TF system or TF tube (room temperature) (1-5 samples per pool) |
2-3 working days |
| (Check guidelines for Bovine Tritrichomonas foetus testing listed above) | ||||
| Tritrichomonas and Campylobacter venerialis Duplex | M143b | $50.00 | In-Pouch TF system or TF tube (room temperature) | 2-3 working days |
| PANELS: | ||||
| Bovine Resp. Panel- Viral PCR (BRSV, BVD, PI-3 and BHV-1) |
M301 | $60.00 | Nasopharyngeal swab, nasal swab, 1-2mL transtracheal wash or bronchoalveolar lavage, 1 gram fresh or frozen lung or trachea | 3 working days |
| Equine Respiratory Panel PCR (EIV, EHV 1 & 4, A. Culture) |
M302 | $100 | Nasal swab (must submit 2 - one in transport media (Amies, Stuarts, etc) and another with 1 mL of sterile saline red-top OR 2 red-top tubes. Fresh tissue lung or trachea Antibiotic Sensitivity $18/ea addl. |
3 working days (Aerobic 2-5 days) |
| *Tests not listed will be forwarded to a referral laboratory for testing. $35.00 S&H will be added to the outside laboratory price. | ||||
For USDA foreign animal disease or State Veterinarian surveillance:
|
Al Realtime PCR Avian Paramyxovirus-1 PCR |
- |
50.00 50.00
|
Cloacal or tracheal swab, tissue (Wild Bird: pooled cloacal/oropharyngeal swab) |
- |
|
African Swine Fever Realtime PCR Classical Swine Fever Realtime PCR Foot and Mouth Disease Realtime PCR |
- |
50.00 50.00 50.00
|
Spleen, tonsil, lymph node tissue Spleen, tonsil, lymph node tissue Oral swab (FMD preferred specimen)
|
- |
Submission Form and User Guide or call us (520) 621-2356 to receive a copy via e-mail or fax.
Published pricing revised May 2023. Price and turnaround time are subject to change at any time