Bacteriology

Proper selection and preservation of samples for bacteriologic analysis is essential for accurate and efficient diagnosis.  Postmortem autolysis of tissues is the most common cause of interference with bacteriologic diagnosis.  Tissues should be very fresh, collected aseptically and immediately refrigerated, and sent by overnight transport.  When prompt submission is not possible, freezing is acceptable for most types of bacteriologic tests, although they are often unsuitable for other microbiologic or pathologic testing.  A second cause of interference is collection of samples too late in the course of the disease or after the initiation of antibiotic therapy.  Submit generous samples of tissue, exudates, or feces.  Avoid the use of swabs when fresh tissues or exudates are available.  The prompt collection of specimens with minimal exposure to air is particularly important for anaerobic culture.  Tissue samples should be submitted in separate containers and properly identified.  Adequate refrigeration in insulated containers with sufficient numbers of ice packs is essential for shipping specimens.  Call the laboratory (520) 621-2356 for any questions on specimen collection, the availability of a test, or to inform us of the impending shipping of large numbers of samples.

 

Acceptable samples for Bacteriological Culture are:

  • All bacteriological cultures:  Specimens should be shipped overnight. Specimens for most bacterial cultures can be frozen, but freezing will interfere with histopathologic examination, and sometimes with other microbiologic tests.
  • Aerobic cultures: Tissues, fluids, or exudates are preferred. If using swabs, submit swabs with aerobic bacterial transport medium: Stuart or Amies (with or without charcoal).
  • Anaerobic cultures: Submit tissues, fluids, or exudates whenever possible.  Avoid excessive exposure to oxygen. Swabs should be used with anaerobic bacterial transport medium (Port-A-Cul or similar). Swabs with Stuart or Amies medium are not suitable for the isolation of strict anaerobes.
  • Campylobacter fetus subsp. venerealis (bovine preputial and vaginal swabs)):  Cary-Blair swabs must be received within 24 hours of collection.
  • Moraxella bovis (Pink eye) culture: Ocular swabs or inoculated blood agar plates (recommended) must be received within 12 hours of collection.
  • Bordetella bronchiseptica cultures from live animals: Nasal or tracheobronchial swabs must be received within 12 hours of collection.
  • Clostridial culture/FA:  Muscle, liver, or other parenchymatous organs collected as soon as possible soon after death and immediately refrigerated.

Acceptable samples for Tritrichomonas foetus culture: Bull preputial or cow vaginal secretions collected in In-Pouch transport pouches, maintained at room temperature, and received within 48 hours of collection. (Pouches for PCR can be refrigerated).

Test Description

Test #

Price

Specimen Preferred

Turnaround Time

ACID FAST STAIN

B109

$  8.25

Tissue; feces

1-2 working days

AEROBIC CULTURE

     (Antibiotic sensitivity additional $16.50 per isolate)

B103

$20.75

Culture swab; exudate; tissue

2-5 working days

ANAEROBIC CULTURE

B104

$27.50

Culture swab; exudates; tissue

2-5 working days

ANTIBIOTIC SENSITIVITY (KIRBY-BAUER DISC)

      **$16.50 cost per isolate**

B101

$16.50

Live Organism

2-5 working days

CAMPYLOBACTER CULTURE

B122

$13.75

Swab in Carry-Blair medium; tissue; feces

3-5 working days

CLOSTRIDIUM SP (FA) PER SPECIES (CL. CHAUVOEI FA)

B113

$13.75

Affected muscle tissue

1-2 working days

CLOSTRIDIUM SP (FA) PER SPECIES (CL. SEPTICUM FA)

B114

$13.75

Affected muscle tissue

1-2 working days

CLOSTRIDIUM SP (FA) PER SPECIES (CL. NOVYI FA)

B115

$13.75

Liver tissue

1-2 working days

FUNGAL CULTURE

B106

$27.50

Tissue; skin scrapings; hair

1-2 weeks

GIMENEZ STAIN

B123

$  8.25

Tissue or impression smears

1-2 working days

GRAM STAIN

B111

$  8.25

Feces, tissue, or impression smears

1-2 working days

MYCOPLASMA CULTURE

B108

$16.50

Culture swab, tissue, milk

7-14 working days

SALMONELLA CULTURE

B126

$20.75

Feces, tissue, swab, eggs, 200 g feed

2-5 working days

TRICHOMONAS CULTURE

B112

$  7.00

Preputial or vaginal secretion in

Trich pouch

3 working days

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